SCAFFOLD KNOT INSTALL
SCAFFOLD KNOT PLUS

Cells displaying a particular epitope sequence could be enriched 10(7)-fold by combining magnetic cell sorting with fluorescence-activated cell sorting. Cells expressing EETI-II variants containing an epitope were shown to be surface labeled with the respective monoclonal antibody by indirect immunofluorescence corroborating the cell surface exposure of the epitope sequences embedded in the EETI-II cystine knot scaffold. To restore cell viability, net accumulation of fusion protein in the outer membrane was reduced to a tolerable level by introduction of an amber codon at position 9 of the lpp' sequence and utilizing an amber suppressor strain as expression host. Cell viability was found to be drastically reduced by high level expression of Lpp'-OmpA'-EETI-II fusion protein. Gene expression was under control of the strong and tightly regulated tetA promoter/operator.

SCAFFOLD KNOT PLUS

EETI-II and derived variants could be presented on the Escherichia coli outer membrane by fusion to truncated Lpp'-OmpA', which comprises the first nine residues of mature lipoprotein plus the membrane spanning beta-strand from residues 46-66 of OmpA protein. By secretion of the fusion into the periplasmic space, fully oxidized and correctly folded EETI-II was obtained in high yield. EETI-II and derived variants were produced via fusion to maltose binding protein MalE. In order to explore the potential of the EETI-II peptide to serve as a structural scaffold for the presentation of randomized oligopeptides, we constructed two EETI-II derivatives, where the six-residue inhibitor loop was replaced by a 13-residue epitope of Sendai virus L-protein and by a 17-residue epitope from human bone Gla-protein. Its compact structure is defined by a triple-stranded antiparallel beta-sheet, which is held together by three intramolecular disulfide bonds forming a cystine knot. Feed the rope or cable through the jaws and around the wrapping area.The Ecballium elaterium trypsin inhibitor II (EETI-II), a member of the squash family of protease inhibitors, is composed of 28 amino acid residues and is a potent inhibitor of trypsin.

SCAFFOLD KNOT INSTALL

Install the invention over the upper and lower rails of a handrail. They are simply wrapped through the jaw and around the hooks like the cord on a vacuum cleaner. With this device there is also no longer a need to tie off the cables. If the rope is accidentally released the jaw mechanism will prevent the items from falling. The benefits of using such a device:Īs articles are being lifted, a jaw like mechanism keeps the rope under control at all times. It eliminates the need for knot training and simplifies the tie-off process. This invention provides a failsafe to prevent dropped items even in the lifting process. There is also a possibility that the ropes could be dropped as the articles are being lifted up to the platform. As a result of improper training the ropes may be tied too tight, resulting in difficult to release situations or the ropes may be tied too loosely, leaving a potential hazard.

Workers are not always trained in the proper ways to tie-off equipment. It was created because:Ī lot of the time in overhead construction, articles are tied to hand rails. This item is patented so making, using, offering for sale, importing, or selling the invention without permission is prohibited. It is an invention that allows workers to tie off ropes for use in scaffold-related work or when articles are tied over rails. A rail mounted tie-off device that eliminates much of the need for knots and hitches.

Scaffold knot